Fig 1: (A) Agarose gel electrophoresis of fragile X. Example applied to patients (DNA 01 and DNA 02). Lane 1 (MM): 50-bp DNA Step Ladder, Lane 2: Healthy Control (HC), Lane 3 (DNA 01): Healthy patient, Lane 4 (DNA 02): Affected patient, Lane 5: Positive Control (PC) and Lane 6: Negative Control (CN). (B,C) Amplification of genetic material by Real-Time PCR of DNA 02 and Positive Control respectively; DNA 02 and Positive control were quantified in Quantus Fluorometer (Promega, Wisconsin, USA) and concentration values between 65 and 75 ng/μl were obtained.
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